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dna employing superscript iii reverse transcriptase  (Vazyme Biotech Co)


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    Vazyme Biotech Co dna employing superscript iii reverse transcriptase
    Dna Employing Superscript Iii Reverse Transcriptase, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 99/100, based on 2179 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+reverse+transcriptase+iii/HiScript+III+All-in-one+RT+SuperMix+Perfect+for+qPCR/pm41991537-302-7-13
    Average 99 stars, based on 2179 article reviews
    dna employing superscript iii reverse transcriptase - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    cDNA Synthesis:

    Article Title: Suppression of ethylene metabolism serves as a key factor underlying high temperature-induced promotion of anthocyanin synthesis of peach skin
    Article Snippet: This study investigated the effects of high temperature (HT, 35C) on anthocyanin accumulation and ethylene metabolism in postharvest ‘Baifeng’ peaches, compared with control temperature (CT, 25C).. Results showed that HT significantly enhanced total anthocyanin content in peach skin by 1.55-fold at day 9, primarily attributed to upregulated expression of biosynthesis genes and transcription factors, as well as increased activities of key enzymes (PAL, CHS, UFGT).. Simultaneously, HT suppressed ethylene production to 12.18 % of CT levels by inhibiting the activities and gene expression of ethylene biosynthesis enzymes and downregulating ethylene signaling pathway.

    Reverse Transcription:

    Article Title: Suppression of ethylene metabolism serves as a key factor underlying high temperature-induced promotion of anthocyanin synthesis of peach skin
    Article Snippet: This study investigated the effects of high temperature (HT, 35C) on anthocyanin accumulation and ethylene metabolism in postharvest ‘Baifeng’ peaches, compared with control temperature (CT, 25C).. Results showed that HT significantly enhanced total anthocyanin content in peach skin by 1.55-fold at day 9, primarily attributed to upregulated expression of biosynthesis genes and transcription factors, as well as increased activities of key enzymes (PAL, CHS, UFGT).. Simultaneously, HT suppressed ethylene production to 12.18 % of CT levels by inhibiting the activities and gene expression of ethylene biosynthesis enzymes and downregulating ethylene signaling pathway.

    Article Title: Regulation of lipid droplets accumulation by the Hippo-YAP/COX2 signaling pathway in neomycin-induced ototoxicity.
    Article Snippet: .. Subsequently, total RNA was reverse-transcribed into complementary DNA employing Superscript III reverse transcriptase (Vazyme, R333). .. Quantitative polymerase chain reaction AR TI CL E IN P RE SS analyses were conducted using an ABI 7500 Real-Time PCR System (Applied Biosystems, Foster City, CA, USA).

    Article Title: Tumor-associated macrophages promote chemoresistance to Paclitaxel via activating NOTCH2-JAG1 juxtacrine signaling.
    Article Snippet: .. Briefly, total RNA was extracted using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol, and RNA concentration AR TIC LE IN PR ES S was determined with a NanoDrop spectrophotometer (Life Technologies, Carlsbad, CA, USA). cDNA was synthesized from 1 g RNA using oligo(dT) primers and Superscript III reverse transcriptase (Vazyme, Nanjing, China). .. Quantitative PCR was carried out using SYBR® Premix (Vazyme, Nanjing, China) on a PikoReal 96 real-time PCR system (Thermo Scientific, MA, USA).

    Article Title: OsBIR3 maintains the homeostasis of OsBRI1, OsREM4.1, and Brd2 protein levels in brassinosteroid pathways in rice.
    Article Snippet: Total rice RNA was extracted from the roots, stems, leaf blades, leaf sheaths, and young panicles with a total RNA isolater kit (R401-01; Vazyme, Nanjing, China). .. The first-strand cDNA was synthesized from total RNA (2 μg) using SuperScript III Reverse Transcriptase Kit (R323-01; Vazyme). qRT-PCR analysis was performed on a CFX96 real-time PCR system (qTOWER3 G; Jena, Germany). .. The amplification was performed in a total volume of 10 μL with 0.1 μM of each primer and 1 × SYBR green qPCR master mix (Q711-03; Vazyme), and the Actin1 gene was used as the internal control.

    Article Title: OsBIR3 maintains the homeostasis of OsBRI1 , OsREM4 .1, and Brd2 protein levels in brassinosteroid pathways in rice
    Article Snippet: Total rice RNA was extracted from the roots, stems, leaf blades, leaf sheaths, and young panicles with a total RNA isolater kit (R401‐01; Vazyme, Nanjing, China). .. The first‐strand cDNA was synthesized from total RNA (2 μg) using SuperScript III Reverse Transcriptase Kit (R323‐01; Vazyme). qRT‐PCR analysis was performed on a CFX96 real‐time PCR system (qTOWER 3 G; Jena, Germany). .. The amplification was performed in a total volume of 10 μL with 0.1 μ m of each primer and 1 × SYBR green qPCR master mix (Q711‐03; Vazyme), and the Actin1 gene was used as the internal control.

    Article Title: Therapeutic Black Phosphorus Nanosheets Elicit Neutrophil Response for Enhanced Tumor Suppression.
    Article Snippet: Advanced Science published by Wiley-VCH GmbH nloaded from https://advanced.onlinelibrary.w iley.com /doi/10.1002/advs.202414779 by U niversity O f T he Philippines, W iley O nline L ibrary on [26/01/2025]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense cDNA was synthesized from 1 μg of RNA using Oligo(dT) primers and Superscript III reverse transcriptase, following the manufacturer’s protocol (Vazyme, China). .. Quantitative PCR was carried out using SYBR Premix (Vazyme, China).

    Concentration Assay:

    Article Title: Tumor-associated macrophages promote chemoresistance to Paclitaxel via activating NOTCH2-JAG1 juxtacrine signaling.
    Article Snippet: .. Briefly, total RNA was extracted using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol, and RNA concentration AR TIC LE IN PR ES S was determined with a NanoDrop spectrophotometer (Life Technologies, Carlsbad, CA, USA). cDNA was synthesized from 1 g RNA using oligo(dT) primers and Superscript III reverse transcriptase (Vazyme, Nanjing, China). .. Quantitative PCR was carried out using SYBR® Premix (Vazyme, Nanjing, China) on a PikoReal 96 real-time PCR system (Thermo Scientific, MA, USA).

    Spectrophotometry:

    Article Title: Tumor-associated macrophages promote chemoresistance to Paclitaxel via activating NOTCH2-JAG1 juxtacrine signaling.
    Article Snippet: .. Briefly, total RNA was extracted using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol, and RNA concentration AR TIC LE IN PR ES S was determined with a NanoDrop spectrophotometer (Life Technologies, Carlsbad, CA, USA). cDNA was synthesized from 1 g RNA using oligo(dT) primers and Superscript III reverse transcriptase (Vazyme, Nanjing, China). .. Quantitative PCR was carried out using SYBR® Premix (Vazyme, Nanjing, China) on a PikoReal 96 real-time PCR system (Thermo Scientific, MA, USA).

    Synthesized:

    Article Title: Tumor-associated macrophages promote chemoresistance to Paclitaxel via activating NOTCH2-JAG1 juxtacrine signaling.
    Article Snippet: .. Briefly, total RNA was extracted using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol, and RNA concentration AR TIC LE IN PR ES S was determined with a NanoDrop spectrophotometer (Life Technologies, Carlsbad, CA, USA). cDNA was synthesized from 1 g RNA using oligo(dT) primers and Superscript III reverse transcriptase (Vazyme, Nanjing, China). .. Quantitative PCR was carried out using SYBR® Premix (Vazyme, Nanjing, China) on a PikoReal 96 real-time PCR system (Thermo Scientific, MA, USA).

    Article Title: OsBIR3 maintains the homeostasis of OsBRI1, OsREM4.1, and Brd2 protein levels in brassinosteroid pathways in rice.
    Article Snippet: Total rice RNA was extracted from the roots, stems, leaf blades, leaf sheaths, and young panicles with a total RNA isolater kit (R401-01; Vazyme, Nanjing, China). .. The first-strand cDNA was synthesized from total RNA (2 μg) using SuperScript III Reverse Transcriptase Kit (R323-01; Vazyme). qRT-PCR analysis was performed on a CFX96 real-time PCR system (qTOWER3 G; Jena, Germany). .. The amplification was performed in a total volume of 10 μL with 0.1 μM of each primer and 1 × SYBR green qPCR master mix (Q711-03; Vazyme), and the Actin1 gene was used as the internal control.

    Article Title: OsBIR3 maintains the homeostasis of OsBRI1 , OsREM4 .1, and Brd2 protein levels in brassinosteroid pathways in rice
    Article Snippet: Total rice RNA was extracted from the roots, stems, leaf blades, leaf sheaths, and young panicles with a total RNA isolater kit (R401‐01; Vazyme, Nanjing, China). .. The first‐strand cDNA was synthesized from total RNA (2 μg) using SuperScript III Reverse Transcriptase Kit (R323‐01; Vazyme). qRT‐PCR analysis was performed on a CFX96 real‐time PCR system (qTOWER 3 G; Jena, Germany). .. The amplification was performed in a total volume of 10 μL with 0.1 μ m of each primer and 1 × SYBR green qPCR master mix (Q711‐03; Vazyme), and the Actin1 gene was used as the internal control.

    Article Title: NR4A3high mast cells promote ovarian cancer metastasis by reprogramming tumor-associated macrophages via JAK2/STAT6 signaling
    Article Snippet: Total RNA was extracted with TRIzol reagent (Vazyme Biotech) according to the manufacturer’s protocol, dissolved in RNA-free ddH 2 O, and stored at –80 °C. .. Then complementary DNA was synthesized from total RNA using Superscript III transcriptase (Vazyme Biotech). .. RT-qPCR was performed using SYBR green (Vazyme Biotech) on a real-time detector (Q5) to determine the mRNA expression levels of genes of interest. β-ACTIN mRNA expression was used to normalize the expression of target genes in each sample.

    Article Title: Therapeutic Black Phosphorus Nanosheets Elicit Neutrophil Response for Enhanced Tumor Suppression.
    Article Snippet: Advanced Science published by Wiley-VCH GmbH nloaded from https://advanced.onlinelibrary.w iley.com /doi/10.1002/advs.202414779 by U niversity O f T he Philippines, W iley O nline L ibrary on [26/01/2025]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense cDNA was synthesized from 1 μg of RNA using Oligo(dT) primers and Superscript III reverse transcriptase, following the manufacturer’s protocol (Vazyme, China). .. Quantitative PCR was carried out using SYBR Premix (Vazyme, China).

    Quantitative RT-PCR:

    Article Title: OsBIR3 maintains the homeostasis of OsBRI1, OsREM4.1, and Brd2 protein levels in brassinosteroid pathways in rice.
    Article Snippet: Total rice RNA was extracted from the roots, stems, leaf blades, leaf sheaths, and young panicles with a total RNA isolater kit (R401-01; Vazyme, Nanjing, China). .. The first-strand cDNA was synthesized from total RNA (2 μg) using SuperScript III Reverse Transcriptase Kit (R323-01; Vazyme). qRT-PCR analysis was performed on a CFX96 real-time PCR system (qTOWER3 G; Jena, Germany). .. The amplification was performed in a total volume of 10 μL with 0.1 μM of each primer and 1 × SYBR green qPCR master mix (Q711-03; Vazyme), and the Actin1 gene was used as the internal control.

    Real-time Polymerase Chain Reaction:

    Article Title: OsBIR3 maintains the homeostasis of OsBRI1, OsREM4.1, and Brd2 protein levels in brassinosteroid pathways in rice.
    Article Snippet: Total rice RNA was extracted from the roots, stems, leaf blades, leaf sheaths, and young panicles with a total RNA isolater kit (R401-01; Vazyme, Nanjing, China). .. The first-strand cDNA was synthesized from total RNA (2 μg) using SuperScript III Reverse Transcriptase Kit (R323-01; Vazyme). qRT-PCR analysis was performed on a CFX96 real-time PCR system (qTOWER3 G; Jena, Germany). .. The amplification was performed in a total volume of 10 μL with 0.1 μM of each primer and 1 × SYBR green qPCR master mix (Q711-03; Vazyme), and the Actin1 gene was used as the internal control.

    Article Title: OsBIR3 maintains the homeostasis of OsBRI1 , OsREM4 .1, and Brd2 protein levels in brassinosteroid pathways in rice
    Article Snippet: Total rice RNA was extracted from the roots, stems, leaf blades, leaf sheaths, and young panicles with a total RNA isolater kit (R401‐01; Vazyme, Nanjing, China). .. The first‐strand cDNA was synthesized from total RNA (2 μg) using SuperScript III Reverse Transcriptase Kit (R323‐01; Vazyme). qRT‐PCR analysis was performed on a CFX96 real‐time PCR system (qTOWER 3 G; Jena, Germany). .. The amplification was performed in a total volume of 10 μL with 0.1 μ m of each primer and 1 × SYBR green qPCR master mix (Q711‐03; Vazyme), and the Actin1 gene was used as the internal control.



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